Lipoprotein fractionation in deuterium oxide gradients: a procedure for evaluation of antioxidant binding and susceptibility to oxidation.
C Hallberg, M Hådén, M Bergström, G Hanson… - Journal of lipid …, 1994 - ASBMB
Oxidative modifications of lipoproteins appear to contribute to their atherogenicity. Very low
and low density lipoproteins (VLDL and LDL) are protected against these modifications by
antioxidants that can be incorporated in vivo or in vitro into the particles. We describe here
ultracentrifugal procedures for isolation of VLDL and LDL that do not require subsequent
dialysis or buffer equilibration. Lipoproteins were isolated in buffers with physiological ionic
composition prepared in D2O (deuterium oxide). This allowed measurements of the content …
and low density lipoproteins (VLDL and LDL) are protected against these modifications by
antioxidants that can be incorporated in vivo or in vitro into the particles. We describe here
ultracentrifugal procedures for isolation of VLDL and LDL that do not require subsequent
dialysis or buffer equilibration. Lipoproteins were isolated in buffers with physiological ionic
composition prepared in D2O (deuterium oxide). This allowed measurements of the content …