Prostacyclin synthase catalyzes the conversion of prostaglandin H2 to prostacyclin, which is
a powerful vasodilator and the most potent natural occurring inhibitor of platelet aggregation.
In the present study, we determined the amino acid sequence of bovine prostacyclin
synthase by combined protein chemical and molecular cloning techniques. The enzyme was
purified and characterized from bovine aorta microsomes, and the partial amino acid
sequences were determined with the native enzyme and endoproteinase Lys-C-cleaved …