[HTML][HTML] Enhanced Levels of Lipoperoxides in Low Density Lipoprotein Incubated with Murine Fibroblasts Expressing High Levels of Human 15-Lipoxygenase (∗)
DJ Benz, M Mol, M Ezaki, N Mori-Ito, I Zelán… - Journal of Biological …, 1995 - ASBMB
There is strong experimental evidence that oxidized low density lipoprotein (Ox-LDL) plays
an important role in atherosclerosis. However, the mechanisms by which Ox-LDL is formed
in vivo are unknown. To test whether 15-lipoxygenase (15-LO) could play a role in oxidation
of LDL by cells, we expressed 15-LO activity in murine fibroblasts, which do not normally
have 15-LO activity, and tested their ability to modify LDL. Using a retroviral vector, we
prepared fibroblasts that expressed 2-to 20-fold more 15-LO activity than control fibroblasts …
an important role in atherosclerosis. However, the mechanisms by which Ox-LDL is formed
in vivo are unknown. To test whether 15-lipoxygenase (15-LO) could play a role in oxidation
of LDL by cells, we expressed 15-LO activity in murine fibroblasts, which do not normally
have 15-LO activity, and tested their ability to modify LDL. Using a retroviral vector, we
prepared fibroblasts that expressed 2-to 20-fold more 15-LO activity than control fibroblasts …