Attenuation of TRPV1 by AMG-517 after nerve injury promotes peripheral axonal regeneration in rats
J Bai, F Liu, LF Wu, YF Wang, XQ Li - Molecular pain, 2018 - journals.sagepub.com
J Bai, F Liu, LF Wu, YF Wang, XQ Li
Molecular pain, 2018•journals.sagepub.comAims The main objective was to investigate the effects of the transient receptor potential
cation channel subfamily V member 1 (TRPV1) on nerve regeneration following sciatic
transection injury by functional blockage of TRPV1 using AMG-517, a specific blocker of
TRPV1. Methods AMG-517 was injected into the area surrounding ipsilateral lumbar dorsal
root ganglia 30 min after unilateral sciatic nerve transection. The number of sciatic axons
and the expression of growth-associated protein-43 (GAP-43) and glial fibrillary acidic …
cation channel subfamily V member 1 (TRPV1) on nerve regeneration following sciatic
transection injury by functional blockage of TRPV1 using AMG-517, a specific blocker of
TRPV1. Methods AMG-517 was injected into the area surrounding ipsilateral lumbar dorsal
root ganglia 30 min after unilateral sciatic nerve transection. The number of sciatic axons
and the expression of growth-associated protein-43 (GAP-43) and glial fibrillary acidic …
Aims
The main objective was to investigate the effects of the transient receptor potential cation channel subfamily V member 1 (TRPV1) on nerve regeneration following sciatic transection injury by functional blockage of TRPV1 using AMG-517, a specific blocker of TRPV1.
Methods
AMG-517 was injected into the area surrounding ipsilateral lumbar dorsal root ganglia 30 min after unilateral sciatic nerve transection. The number of sciatic axons and the expression of growth-associated protein-43 (GAP-43) and glial fibrillary acidic protein was examined using semithin sections, Western blot, and immunofluorescence analyses.
Results
Blockage of TRPV1 with AMG-517 markedly promoted axonal regeneration, especially at two weeks after sciatic injury; the number of axons was similar to the uninjured control group. After sciatic nerve transection, expression of glial fibrillary acidic protein was decreased and GAP-43 was increased at the proximal stump. However, the expression of both glial fibrillary acidic protein and GAP-43 increased significantly in AMG-517-treated groups.
Conclusions
TRPV1 may be an important therapeutic target to promote peripheral nerve regeneration after injury.
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