Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice
T Christoph, G Bahrenberg, J De Vry… - Molecular and Cellular …, 2008 - Elsevier
The function of the transient receptor potential vanilloid 1 (TRPV1) cation channel was
analyzed with RNA interference technologies and compared to TRPV1 knockout mice.
Expression of shRNAs targeting TRPV1 in transgenic (tg) mice was proven by RNase
protection assays, and TRPV1 downregulation was confirmed by reduced expression of
TRPV1 mRNA and lack of receptor agonist binding in spinal cord membranes.
Unexpectedly, TRPV3 mRNA expression was upregulated in shRNAtg but downregulated in …
analyzed with RNA interference technologies and compared to TRPV1 knockout mice.
Expression of shRNAs targeting TRPV1 in transgenic (tg) mice was proven by RNase
protection assays, and TRPV1 downregulation was confirmed by reduced expression of
TRPV1 mRNA and lack of receptor agonist binding in spinal cord membranes.
Unexpectedly, TRPV3 mRNA expression was upregulated in shRNAtg but downregulated in …