Osmotic pumps continuously deliver compounds at a constant rate into small animals. This
article introduces a standard protocol used to induce aortic aneurysms via subcutaneous
infusion of angiotensin II (AngII) from implanted osmotic pumps. This protocol includes
calculation of AngII amount and dissolution, osmotic pump filling, implantation of osmotic
pumps subcutaneously, observation after pump implantation, and harvest of aortas to
visualize aortic aneurysms in mice. Subcutaneous infusion of AngII through osmotic pumps …