Plasmodium falciparum genetic crosses in a humanized mouse model
Nature methods, 2015•nature.com
Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium
falciparum are a powerful tool for identifying genes controlling drug resistance and other key
phenotypes. Previous studies relied on the isolation of recombinant parasites from
splenectomized chimpanzees, a research avenue that is no longer available. Here we
demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the
identification of genetic determinants of parasite traits and adaptations.
falciparum are a powerful tool for identifying genes controlling drug resistance and other key
phenotypes. Previous studies relied on the isolation of recombinant parasites from
splenectomized chimpanzees, a research avenue that is no longer available. Here we
demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the
identification of genetic determinants of parasite traits and adaptations.
Abstract
Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.
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