Molecular analysis of T cell repertoires: spectratypes generated by multiplex polymerase chain reaction and evaluated by radioactivity or fluorescence
K Maślanka, T Piatek, J Gorski, M Yassai, J Gorski - Human immunology, 1995 - Elsevier
The analysis of T-cell repertoires has been facilitated by the introduction of methods in which
the length heterogeneity of the third complementarity region (CDR3) is used to further refine
V-family-specific PCR. We call our implementation of this technique T-cell spectratyping.
This method is especially important in analysis of spécific expansion or retention of T cells in
human immune system function. Current methodologies are cumbersome in the number of
PCR reactions and gels needed for complete analysis of TCR BV repertoires. We describe …
the length heterogeneity of the third complementarity region (CDR3) is used to further refine
V-family-specific PCR. We call our implementation of this technique T-cell spectratyping.
This method is especially important in analysis of spécific expansion or retention of T cells in
human immune system function. Current methodologies are cumbersome in the number of
PCR reactions and gels needed for complete analysis of TCR BV repertoires. We describe …
