Studies on human platelets. I. synthesis of platelet protein in a cell-free system
FM Booyse, ME Rafelson Jr - Biochimica et Biophysica Acta (BBA)-Nucleic …, 1968 - Elsevier
A reproducible cell-free system, active in protein synthesis, has been prepared from human
blood platelets. The ability of this system to incorporate 14 C-labeled amino acids, is
dependent on the presence of ribosomes, a soluble enzyme fraction (S-150), and ATP plus
an ATP-generating system. Both puromycin and ribonuclease have the ability to inhibit
amino acid incorporation. A major soluble protein product of this system has been
characterized; the solubility properties, sucrose gradient patterns, and fingerprint patterns of …
blood platelets. The ability of this system to incorporate 14 C-labeled amino acids, is
dependent on the presence of ribosomes, a soluble enzyme fraction (S-150), and ATP plus
an ATP-generating system. Both puromycin and ribonuclease have the ability to inhibit
amino acid incorporation. A major soluble protein product of this system has been
characterized; the solubility properties, sucrose gradient patterns, and fingerprint patterns of …