[HTML][HTML] Gene expression profile of primary human CD34+ CD38lo cells differentiating along the megakaryocyte lineage
MH Shim, A Hoover, N Blake, JG Drachman… - Experimental …, 2004 - Elsevier
OBJECTIVE: To identify genes involved in megakaryopoiesis, high-density oligonucleotide
microarrays were used to compare transcript profiles from undifferentiated CD34+ CD38lo
cells and culture-derived megakaryocytes (MKs). MATERIALS AND METHODS:
Megakaryocyte differentiation was achieved in vitro by inducing primary human CD34+
CD38lo cells in serum-deprived media supplemented with the cytokine combination of
interleukin-3, interleukin-6, stem cell factor, and thrombopoietin for 10 days. Three replicate …
microarrays were used to compare transcript profiles from undifferentiated CD34+ CD38lo
cells and culture-derived megakaryocytes (MKs). MATERIALS AND METHODS:
Megakaryocyte differentiation was achieved in vitro by inducing primary human CD34+
CD38lo cells in serum-deprived media supplemented with the cytokine combination of
interleukin-3, interleukin-6, stem cell factor, and thrombopoietin for 10 days. Three replicate …