[HTML][HTML] Increased erythropoiesis in polycythemia vera is associated with increased erythroid progenitor proliferation and increased phosphorylation of Akt/PKB
C Dai, IJ Chung, SB Krantz - Experimental hematology, 2005 - Elsevier
OBJECTIVE: The aim of this study was to explore the mechanism by which increased
erythropoiesis occurs in polycythemia vera (PV). METHODS: CD34+ and erythroid colony-
forming cells (ECFC) were purified from normal or PV peripheral blood and then incubated
in the presence of erythropoietin (EPO) to generate erythroid progenitor cells. Measurement
of proliferation by Ki-67 staining, TUNEL assays to measure apoptosis, and Western blots for
detection of Akt/PKB and glycogen synthase kinase 3 (GSK3) phosphorylation were …
erythropoiesis occurs in polycythemia vera (PV). METHODS: CD34+ and erythroid colony-
forming cells (ECFC) were purified from normal or PV peripheral blood and then incubated
in the presence of erythropoietin (EPO) to generate erythroid progenitor cells. Measurement
of proliferation by Ki-67 staining, TUNEL assays to measure apoptosis, and Western blots for
detection of Akt/PKB and glycogen synthase kinase 3 (GSK3) phosphorylation were …
