We present data demonstrating the gene expression of substance P (SP) and its receptor in
human peripheral blood-isolated lymphocytes. Using reverse transcribed polymerase chain
reaction (RT-PCR) assay, preprotachykinin-A (substance-P) mRNA is detected in human
peripheral blood-isolated lymphocytes. Among the α, β and γ transcripts of the SP gene, only
the β and γ transcripts are detectable in these cells. These RT-PCR amplified transcripts are
recognized by Southern blot assay using a specific SP probe. Direct DNA sequence …