[HTML][HTML] Sensing of extracellular cations in CasR-deficient osteoblasts: evidence for a novel cation-sensing mechanism
M Pi, SC Garner, P Flannery, RF Spurney… - Journal of Biological …, 2000 - ASBMB
We isolated osteoblastic cell lines from wild-type (CasR+/+) and receptor null (CasR−/−)
mice to investigate whetherCasR is present in osteoblasts and accounts for their responses
to extracellular cations. Osteoblasts from bothCasR+/+ and CasR−/− mice displayed an
initial period of cell replication followed by a culture duration-dependent increase in alkaline
phosphatase activity, expression of osteocalcin, and mineralization of extracellular matrix. In
addition, a panel of extracellular cations, including aluminum and the CasR agonists …
mice to investigate whetherCasR is present in osteoblasts and accounts for their responses
to extracellular cations. Osteoblasts from bothCasR+/+ and CasR−/− mice displayed an
initial period of cell replication followed by a culture duration-dependent increase in alkaline
phosphatase activity, expression of osteocalcin, and mineralization of extracellular matrix. In
addition, a panel of extracellular cations, including aluminum and the CasR agonists …
