Thrombin and thrombin receptor agonist peptide induce early events of T cell activation and synergize with TCR cross-linking for CD69 expression and interleukin 2 …
B Mari, V Imbert, N Belhacene, DF Far… - Journal of Biological …, 1994 - ASBMB
Thrombin stimulation of the T leukemic cell line Jurkat induced a transient increase in [Ca2+]
i. Proteolytic activity of the enzyme was required for this effect since diisopropyl
fluorophosphate-thrombin failed to increase [Ca2+] i. Furthermore, hirudin and anti-thrombin
III inhibited the thrombin-induced [Ca2+] i rise in Jurkat T cells. A synthetic thrombin receptor
agonist peptide (TRP) of 7 residues (SFLLRNP) was found to be as effective as thrombin for
[Ca2+] i mobilization, and both agonists induced Ca2+ release exclusively from internal …
i. Proteolytic activity of the enzyme was required for this effect since diisopropyl
fluorophosphate-thrombin failed to increase [Ca2+] i. Furthermore, hirudin and anti-thrombin
III inhibited the thrombin-induced [Ca2+] i rise in Jurkat T cells. A synthetic thrombin receptor
agonist peptide (TRP) of 7 residues (SFLLRNP) was found to be as effective as thrombin for
[Ca2+] i mobilization, and both agonists induced Ca2+ release exclusively from internal …
